Figure 5.

RGD binding analysis and cellular targets. (a) [⁶⁸Ga]-RGD imaging protocol. (b) Representative MIP PET/CT [⁶⁸Ga]-RGD scans (upper panel, non-perfusion-corrected), quantification by VOI analysis, cumulative average ratios of perfusion-normalized radiotracer uptake, n=6 (d0); n=11 (d4), n=10 (d10) and n=9 (d20). * vs. baseline, Kruskal-Wallis test. The color bar represents the color spectrum scaled to the middle VOI of the control leg and indicates the ischemic to non-ischemic ratio. (c-d) RGD-binding analysis by flow cytometry with CruzFluor750-labeled RGD peptide in single cell suspensions from muscles. (c) Representative flow cytometry of RGD-CF750. (d) Relative frequencies of cell populations in total RGD-CF750⁺ gate, pooled from 6 independent experiments. n=5 (d0), 6 (d4), 8 (d10). *P<0.05, **P<0.01 ***P<0.001, one-way ANOVA with Bonferroni's multiple comparison test.