Table 2.
Pneumococcal resistance featuresA found within non-pneumococcal strains carrying homologs of pneumococcal cps loci.
| Non-pneumococcal | ||||||||
|---|---|---|---|---|---|---|---|---|
| Isolate (cps type) | species | MICsB (μg/ml) |
other resistance determinantsA | MICsB |
||||
| Pen | Cft | ery | cli | cot | tet | |||
| KE67013 (5) | S. mitis | =0.5 | = 0.25 | FolA(I100L)/FolPins181, tetM | ≤0.03 | ≤ 0.03 | =4 | > 8 |
| US0049 (5) | S. oralis | ≤0.03 | = 0.12 | negative | ≤0.03 | ≤ 0.03 | ≤0.12 | ≤ 0.25 |
| US0024 (5) | S. infantis | =0.5 | =0.5 | mef/msrD | =2 | ≤0.03 | ≤ 0.12 | = 0.5 |
| US969j1 (5) | S. infantis | = 0.06 | = 0.06 | negative | ≤0.03 | ≤ 0.03 | = 0.25 | = 0.5 |
| KE67213 (12) | S. oralis | =0.25 | = 0.25 | FolA(I100L), tetM | ≤0.03 | ≤ 0.03 | =2 | = 4 |
| KE66813 (15A/F) | S. oralis | =0.5 | =0.5 | FolA(I100L) | ≤0.03 | = 0.06 | =4 | = 0.5 |
| KE66913 (33) | S. oralis | = 0.06 | = 0.12 | FolA(I100L) | ≤0.03 | ≤ 0.03 | =4 | = 0.5 |
| KE66713 (18) | S. mitis | =0.12 | = 0.12 | FolA(I100L)/FolPins181 | ≤0.03 | ≤ 0.03 | =4 | = 0.5 |
| L006 (1)C,D | S. mitis | =1 | =1 | ermB, FolA (I100L), tetM | > 32 | > 2 | =4 | = 4 |
| L164 (1)C,D | S. mitis | =2 | =1 | ermB, FolA (I100L), tetM | > 32 | > 2 | =2 | = 8 |
| L115 (1)C,D | S. mitis | =2 | =1 | mef/msrD, FolA(I100L)/FolPins178, tetM | = 4 | ≤0.03 | =4 | > 8 |
| L116 (1)C,D | S. mitis | =2 | =1 | mef/msrD, FolA(I100L)/FolPins178, tetM | = 4 | ≤0.03 | =4 | > 8 |
| L121 (1)C | S. mitis | =0.12 | = 0.06 | ermB, mef/msrD, tetM | > 32 | > 2 | ≤0.12 | > 8 |
AWGS pipeline features and abbreviations of resistance determinants as described (Metcalf et al., 2016a,b). BMinimum inhibitory concentrations determined conventionally and MICs indicated in red are indicative for non-susceptibility within pneumococci according to previously described parameters (Metcalf et al., 2016a). pen, penicillin; ery, erythromycin; cli, clindamycin; cot, cotrimoxazole; tet, tetracycline. CThese five strains have been previously described (Lessa et al., 2018) and include two separate pairs of highly related strains (each indicated in blue or green font). These strains were recovered from the same US carriage study as strains US0049, US0024, and US969j1 that are included in this study and table. DThe two highly related isolates L115 and L116 shared identical PBP types with both contained penicillin binding protein subtype PBP2X-8 commonly found within penicillin-non-susceptible pneumococci (Metcalf et al., 2016b; Beall et al., 2018). Highly related strains L006 and L164 shared 2 of the 3 PBP subtypes that define the PBP type. Of the 7 S. mitis included, only L121 did not yield an identifiable PBP type through the pneumococcal WGS resistance pipeline.