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. 2019 Jan 8;9:3014. doi: 10.3389/fmicb.2018.03014

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Copyright © 2019 Shi, Cavagnino, Rabefiraisana, Lazar, Li de la Sierra-Gallay, Ochsenbein, Valerio-Lepiniec, Urvoas, Minard, Mijakovic and Nessler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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DNA-binding activity of the short and long forms of the bE8-YabT(∆TM) fusion protein. 0.075 μM dsDNA (210 bp) (A) and 0.15 μM ssDNA (90 nt) (B) were incubated at 310 K for 30 min with 0 μM (lane 1, 5 and 9), 0.4 μM (lane 2, 6 and 10), 0.8 μM (lane 3, 7 and 11), and 1.2 μM (lane 4, 8 and 12) of protein, respectively. The samples were then submitted to electrophoretic mobility shift assays (EMSA). The activity of YabT(∆TM) is used as positive control.