Table 2.
Electrophysiological parameters of human cortical pyramidal cells and experimental protocols related to patient and cell codes.
| Parameter/experiment | Median | IQR | Cell # | Patient/cell codes |
|---|---|---|---|---|
| Vm rest (mV) | -91.6 | -89.3 – -93 | n = 10 | A1, B4–B6, C7, D8–D12 |
| Rin (MΩ) | 160.3 | 123 – 195.5 | n = 11 | A1, B4–B6, C7, D8–D13 |
| Membrane tau (ms) | 22.6 | 19.2 – 28 | n = 11 | A1, B4–B6, C7, D8–D13 |
| Capacitance (pF) | 145.7 | 137.9 – 188 | n = 11 | A1, B4–B6, C7, D8–D13 |
| Rheobase current (pA) | 85 | 39 – 154 | n = 11 | A1, B4–B6, C7, D8–D13 |
| Spike threshold (mV) | -55.8 | -48.5 – -61.3 | n = 11 | A1, B4–B6, C7, D8–D13 |
| Spike amplitude (mV) | 90.4 | 87.6 – 91.9 | n = 11 | A1, B4–B6, C7, D8–D13 |
| Spike half-width (ms) | 1.1 | 1 – 1.2 | n = 11 | A1, B4–B6, C7, D8–D13 |
| AHPfast (mV) | 12.7 | 7.2 – 14.5 | n = 11 | A1, B4–B6, C7, D8–D13 |
| Sag ratio (-100 pA) | 0.882 | 0.864 – 0.904 | n = 9 | A1, B5, C7, D8–D13 |
| Rebound amplitude (-100 pA; mV) | 1.9 | 1.3 – 3.5 | n = 9 | A1, B5, C7, D8–D13 |
| Instantaneous firing rate (Hz) | 14 | 12 – 17.5 | n = 11 | A1, B4–B6, C7, D8–D13 |
| Adaptation index | 0.233 | 0.189 – 0.322 | n = 11 | A1, B4–B6, C7, D8–D13 |
| sEPSC frequency (Hz) | 2.4 | 1.4 – 8.2 | n = 10 | A1, B2–B4, C8, D9, D12, E16–E18 |
| sEPSC amplitude (pA) | 13.2 | 8 – 15.6 | n = 10 | A1, B2–B4, C8, D9, D12, E16–E18 |
| mEPSC frequency (Hz) | 1.7 | 1.6 – 3.3 | n = 9 | D13, D14, E15, F19–F21, G23–G25 |
| mEPSC amplitude (pA) | 8.6 | 8.1 – 10.2 | n = 9 | D13, D14, E15, F19–F21, G23–G25 |
| LY354740 (1 μM) tested on sEPSCs | – | – | n = 6 | A1, B2–B4, C8, E16 |
| LY354740 (0.1 μM) tested on sEPSCs | – | – | n = 2 | D9, D12 |
| CPPG (0.1 mM) + LY354740 (1 μM) tested on sEPSCs | – | – | n = 6 | F22, H26–H30 |
| LY354740 (1 μM) tested on mEPSCs | – | – | n = 6 | F19–F21, G23–G25 |
| LY354740 (0.1 μM) tested on mEPSCs | – | – | n = 3 | D13, D14, E15 |
| LY354740 (1 μM) tested on holding current | – | – | n = 11 | A1, B4, C8, E16, E17, F19–F21, G23–G25 |
| LY354740 (0.1 μM) tested on Rin | – | – | n = 5 | D9, D12, D13, D14, E15 |
| LY354740 (1 μM) tested on Rin | – | – | n = 6 | F19, F20, F21, G23, G24, G25 |
| CPPG (0.1 mM) + LY354740 (1 μM) tested on Rin | – | – | n = 6 | F22, H26, H27, H28, H29, H30 |
Vmrest, resting membrane potential; Rin, input resistance; AHPfast, fast afterhyperpolarization; sEPSCs, spontaneous excitatory postsynaptic currents; mEPSCs, miniature excitatory postsynaptic currents; IQR, inter-quartile range.