Scheme for Fmoc-based solid-phase peptide synthesis with ruthenium-catalyzed olefin metathesis. Reactions are numbered by step within the protocol, where Subheading 3.2, step 2 represents the deprotection reaction, Subheading 3.2, step 4 the coupling reaction, and Subheading 3.3 the metathesis reaction. The chemical structure in Subheading 3.2, step 2 is that of piperidine, Subheading 3.2, step 4 the Fmoc-protected amino acid, and Subheading 3.3 the Grubbs catalyst. Gray ball represents rink amide MBHA resin