Figure 5.

Inhibition of miR-643 impairs apoptosis of SW-480 cells interacting with trophozoites. (A) SW-480 cells were or not transfected with miR-643 inhibitor or scramble and interacting with trophozoites for 45 min. Then apoptosis of SW-480 cells was quantified using annexin V assays. SW-480 cells without exposure to E. histolytica and cells treated with cisplatin (50 μM, during 12 h) were used as controls. (B) Protein extracts from miR-643 deficient SW-480 cells interacting with E. histolytica trophozoites for 45 min and controls were submitted to Western blot assays using caspase-3 and caspase-9 antibodies. GADPH antibodies were used as loading control. (C) Immunodetected bands intensity in panel B were quantified by densitometry and normalized to GAPDH. Values are expressed relative to the control group (0 h, interaction). **p < 0.01; ***p < 0.001.