Fig. 9. (A) Zebrafish microangiography by injection of Pttc-TFQ-BODIPY Pdots into 3 days post fertilization Tg(kdrl:eGFP) zebrafish embryos. The global view of zebrafish vessel and Pdot images is shown in left panels (a–c), and a close view of the trunk vasculature (intersegmental vessel, ISV) is shown in the right panels (d–f). Green emission is from endothelial cells expressing eGFP, while red emission is from the Pdots. Scale bars represent 600 μm in (a–c) and 20 μm in (d–f), respectively. (B) Time-dependent in vivo bright-view overlaid with fluorescence imaging of the mouse intravenously injected with folate-functionalized Pdots. (C) Overlap images of fluorescence images and bright-view images of different organs from the mice at 76 h after tail-vein injection of saline (left), bare Pdots (middle), and folate-functionalized Pdots (right). (D) Comparison of biodistribution of the three Pdot probes in the resected organs. Black columns: saline treated mouse; light blue columns: bare Pdot treated mouse; red columns: Pdot–folate treated mouse.