Table 3. Photophysical data of BODIPY-based NIR Pdots in water compared with other water-soluble typical NIR dyes.
Fluorescent probes | λ abs max (nm) | λ em max (nm) | f whm e (nm) | ε max f (M–1 cm–1) | σ g (cm2) | Φ (%) | Brightness h (σ × Φ) (cm2) |
ATTO740 a | 740 | 764 | 43 | 1.20 × 105 | 4.58 × 10–16 | 10 | 4.58 × 10–17 |
Cy 5.5 b | 674 | 694 | 44 | 1.95 × 105 | 7.45 × 10–16 | 23 | 1.71 × 10–16 |
NIR7.0–2 b | 777 | 798 | 50 | 1.20 × 105 | 4.58 × 10–16 | 2.5 | 1.15 × 10–17 |
Qdot 705 c | 405 (473) | 707 | 72 | 8.30 (3.60) × 106 | 3.17 (1.38) × 10–14 | 82 | 2.60 (1.13) × 10–14 |
PF-TFQ-BODIPY | 512 (473) | 723 | 45 | 1.43 (1.14) × 108 | 5.46 (4.35) × 10–13 | 7 | 3.82 (3.05) × 10–14 |
TPE-TFQ-BODIPY d | 493 (473) | 727 | 44 | 1.01 (0.95) × 108 | 3.86 (3.61) × 10–13 | 37 | 1.43 (1.33) × 10–13 |
Pttc-TFQ-BODIPY d | 490 (473) | 724 | 44 | 1.01 (0.97) × 108 | 3.86 (3.71) × 10–13 | 51 | 1.97 (1.89) × 10–13 |
aData from the ATTO-TEC catalog.
bData from C. Bouteiller, G. Clavé, A. Bernardin, B. Chipon, M. Massoneau, P.-Y. Renard, A. Romieu. Bioconjugate Chem. 2007, 18, 1303–1317.
cData from Invitrogen (Thermo Fisher Scientific).
dPdots in deionized water.
eFull width at half maximum.
fMean extinction coefficient.
gMean absorption cross-section.
hMean fluorescence brightness.