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. 2018 Dec 1;29(25):3039–3051. doi: 10.1091/mbc.E18-04-0256

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© 2018 Damodaran, Venkatachalapathy, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

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FIGURE 1: — Compressive force causes chromatin condensation. (A) Experimental setup for Compressive force experiment. (B) Maximum intensity projection of confocal images of nucleus. DNA stained with DAPI (green). (C) Chromatin condensation in percentage based on DAPI stain of the nucleus. N: Rectangle (C^# = 83, L^# = 83); Circle (C^# = 93 and L^# = 90). (D) Maximum intensity projection of confocal images of nucleus. DNA stained with DAPI (green). (E) Chromatin condensation in percentage based on DAPI stain of the nucleus. C^# = 138, L^# = 144, R^# = 124. Whisker box plotted from 10 to 90 percentile. Student’s t test, ***p < 0.0001, **p < 0.01, *p < 0.05, ns = not significant. Scale bar: 5 µm. #C = control; L = load, and R = load + recovery.