Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 1;29(25):3039–3051. doi: 10.1091/mbc.E18-04-0256

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Damodaran, Venkatachalapathy, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

PMC Copyright notice

FIGURE 3: — HDAC3 is a critical regulator of compressive force–induced heterochromatin. (A) Maximum intensity projection of confocal images of cell. DNA stained with DAPI (green) and IF staining of HDAC3 (red). Nuc = nucleus fraction and Cyt = cytoplasmic fraction (B) Nuclear to total protein ratio of HDAC3 based on IF staining. N: C^# = 124, L^# = 132. (C) Maximum intensity projection of confocal images of a nucleus from cells treated with Trichostatin A. DNA stained with DAPI (green). Scale bar: 5 µm. (D) Chromatin condensation in percentage based on DAPI stain of the nucleus. N: C^# = 33, TSA_ C^# = 55, TSA_ L^# = 53. (E) Maximum intensity projection of confocal images of cell. DNA stained with DAPI (green) and IF staining of HDAC3 (red). Si-C = Cells transfected with control siRNA and si-H3 = cells transfected with siHDAC3. (F) Chromatin condensation in percentage based on DAPI stain of the nucleus. N: si-C_C = 96, si-C_L = 125, si-H3_C = 114, si-H3_L = 131. Whisker box plotted from 10 to 90 percentile. Student’s t test, ***p < 0.0001, ns = not significant. Scale bar: 5 µm. #C = control; L = load; TSA_C = TSA-treated control; TSA_L = TSA-treated load; si-C_C = control siRNA-treated rectangle control; si-C_L = control siRNA-treated rectangle load; si-H3_C = HDAC3 siRNA-treated rectangle control; si-H3_L = HDAC3 siRNA-treated rectangle load.