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. Author manuscript; available in PMC: 2019 Jan 15.
Published in final edited form as: Curr Biol. 2017 Aug 31;27(17):2670–2676.e4. doi: 10.1016/j.cub.2017.07.038
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Sheep anti-GFP Wieschaus lab N/A
Rabbit anti-PTyr Santa Cruz Biotechnology Catalog # sc-18182 RRID: AB_670513
Mouse anti-NPC Abeam Catalog # ab24609 RRID: AB_448181
Goat anti-Rabbit IgG Alexa-546 Life Technology Catalog # A11035 RRID: AB_143051
Donkey anti-Sheep IgG Alexa 488 Life Technology Catalog # A11015 RRID: AB_141362
Goat anti-Mouse IgG Alexa-647 Life Technology Catalog # A32728 RRID: AB_2633277
Chemicals, Peptides, and Recombinant Proteins
Phalloidin Alexa-647 Life Technology Catalog # A22287 RRID: AB_2620155
Aqua-Poly/Mount Polysciences Catalog # 18606
Experimental Models: Organisms/Strains
Fly: wild type: Oregon R Bloomington Drosophila Stock Center Stock number: 5
Fly: Resille-GFP Wieschaus lab N/A
Fly: egalitarian: eglwu50 Schüpbach lab N/A
Fly: egalitarian: eglRC12 Schüpbach lab N/A
Software and Algorithms
MATLAB The Mathworks https://www.mathworks.com/
Imaris Bitplane http://www.bitplane.com