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. Author manuscript; available in PMC: 2019 Nov 29.
Published in final edited form as: Cell. 2018 Nov 29;175(6):1520–1532.e15. doi: 10.1016/j.cell.2018.10.043

Figure 5. Zinc-Induced Conformational Rearrangements at the ATD.

Figure 5.

(A) Distance between COMs of the R1 and R2 lobes of GluN2A subunits for the various structural classes. The area shaded in grey represents the open ATD clamshell, and the area shaded in red represents the closed ATD clamshell. The extended-2, splayed-open and super-splayed conformations were not included in this analysis due to poor resolution. Side view of the GluN2A ATD in the closed and open clamshell conformations is shown to the right, with the R2 lobes aligned. The black arrow indicates the direction of motion. * denotes significance (p < 0.05) using a two-tailed homoscedastic student’s t-tests.

(B) Closure of the ATD clamshell increases the distance between the GluN1 and GluN2A R2 lobes. The grey shaded area represents the open GluN2A ATD clamshell, and the red shaded area represents the closed GluN2A ATD clamshells with well-defined density for ATD-LBD linkers. Side view of the GluN1 and GluN2A R2 lobes in the closed and open GluN2A clamshell conformations is shown to the right, with the R2 lobes of GluN1 aligned.

(C) When both ATD clamshells are closed, the GluN2A R2 lobes move away from each other perpendicular to the R1-R2 axis. Top view of the GluN2A ATDs in the 2-knuckle and extended-2 conformations is shown to the right, aligned by the pseudo-symmetry axes. The black arrows indicates the direction of motion.