Fig. 3. Construction of the mutant A. balhimycina_ΔbpsCX. Schematic representation of the deletion of the X domain. Domain arrangement shown for the balhimycin NRPS. NRPS domain descriptions: C, condensation; A, adenylation; PCP, peptidyl carrier protein; E, epimerisation; X, P450 (Oxy) recruitment; TE, thioesterase. ermE, erythromycin resistance gene. bpsCXleft (2079 bp): red; bpsCXright (1916): green. To obtain the deletion mutant A. balhimycina_ΔbpsCX a double crossover via homologous recombination is required.