Table 3. Michaelis–Menten kinetics determined for the different Tcp12 constructs.
Tcp12 construct | Tcp12 concentration [μM] | k cat [min–1] | K m [mM] |
Tcp12 holo-form | 1 | 1.12 ± 0.01 | 0.004 ± 0.0001 |
Tcp12 apo-form | 0.5 | 0.87 ± 0.03 | 0.005 ± 0.0005 |
Tcp12_ΔTE1 | 0.5 | 0.79 ± 0.02 | 0.006 ± 0.0006 |
Tcp12_ΔTE2 | 0.5 | 1.28 ± 0.04 | 0.01 ± 0.0009 |
Tcp12_ΔTE3 | 0.5 | 1.02 ± 0.02 | 0.008 ± 0.0006 |