Table 5. Summary of A-domain rates for Dpg and 4-Hpg and product yields gained from the C-domain activity assay.

Time [min]	Yield a [%]
	k cat [min–1]	Dpg-extended peptide products b	PCP6-bound peptide c	Hydrolysed starting peptide d
Dpg	0.8 ± 0.02	68 ± 0.6	20 ± 0.3	12 ± 0.2
4-Hpg	0.3 ± 0.004	33 ± 2.7	9 ± 1.2	58 ± 3.3
No AA	—	0	28 e ± 0.1	72 e ± 1.7
No AA/Tcp12_ΔTE2	—	—	92 ± 0.5	8 ± 0.5

^aTotal yield of extended peptide is based on the percentage reduction of initial hexapeptide peak from initial starting material.

^bSum of elongated products either cleaved through the use of methylamine to cleave the PCP-bound thioester hydrolysed from the PCP₇ domain of the Tcp12 construct during the course of the reaction.

^cPCP₆-bound peptide substrate cleaved with methylamine.

^dStarting peptide hydrolysed from PCP₆ during the course of the reaction.

^eReaction included co-incubation with OxyB_bal enzyme, so these values also include a very small proportion of monocyclic peptide starting material (<5%).