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. 2018 Aug 16;5(6):1082–1091. doi: 10.1039/c8mh00704g

Fig. 3. CD40mAb released from the PLLA-PDA-CD40mAb scaffold activates DCs from PBMCs. (a) The morphology of DCs and (b) the expressions of CD11c and HLA-DR. Control: cells seeded into culture medium without cytokines and antibodies. MFI: median fluorescence intensity. The proliferation effect of PLLA-PDA-CD40mAb on MC3T3-E1 cells. (c) SEM images of MC3T3-E1 cells seeded onto the electrospun PLLA-PDA, PLLA-PDA-IgG and PLLA-PDA-CD40mAb scaffolds for 3 d. (d) Live/dead staining of MC3T3-E1 cells seeded onto the electrospun PLLA-PDA, PLLA-PDA-IgG and PLLA-PDA-CD40mAb scaffolds for 3 d. (e) CCK-8 assay of MC3T3-E1 cells cultured in the released buffer (24 h) for 1, 3, and 5 d. (f) CCK-8 assay of MC3T3-E1 cells cultured in the released buffer (12, 24, 48 h) for 3 d – A: 12 h, B: 24 h, C: 48 h. Control: MC3T3-E1 cells seeded onto the plate with cell culture medium.

Fig. 3