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. Author manuscript; available in PMC: 2019 Jun 11.
Published in final edited form as: Sci Signal. 2018 Dec 11;11(560):eaat3178. doi: 10.1126/scisignal.aat3178

Figure 3. Migration of DKO T cells is more dependent on myosin activity.

Figure 3.

A and B) CD4+ T cells were allowed to adhere to ICAM-1 coated coverslips for 20 mins, washed, and the percentage of migrating cells was calculated after 5 min pre-treatment with DMSO or with increasing concentrations of (A) Y-27632 or (B) S-nitro-blebbistatin. Data are mean +/− SD from 3 experiments. C) Representative images of migrating CD4+ T cells treated with DMSO, 10μM Y-27632, or 10μM S-nitro-blebbistatin. Cells were fixed and stained with phalloidin. Scale bar, 10μm. D) Length/width ratios of individual cells migrating under the different treatments. Cells that were not migratory were predominantly round and, thus, excluded from the analysis. Data are mean +/− SD from 3 experiments. *p<0.05; **p<0.01; ***p<0.001 by one-way ANOVA for comparison of multiple groups, or by a t-test for comparison of two groups.