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. 2019 Jan 15;14(1):e0210248. doi: 10.1371/journal.pone.0210248

Fig 9. Inhibition of LRRK2 kinase activity attenuates Mn-induced MAPK p38 and ERK, but not JNK in RAW 264.7 and HMC3 cells.

Fig 9

(A,C,E) After pre-treatment with GSK (1 μM) for 90 min, cells were exposed to Mn (250 μM) for 30 min, followed by total protein extraction and western blot analysis as described in the Methods section. (B,D,F) The levels of total and phosphorylated p38 (B), ERK (D) and JNK (F) were quantified. @, p < 0.05; @@, p < 0.01; *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared to the control (one-way ANOVA followed by Tukey’s post hoc test; n = 3). The data shown are representative of 3 independent experiments.