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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Cell. 2018 Nov 1;175(4):921–933.e14. doi: 10.1016/j.cell.2018.10.033

Figure 6. The FHA-2 domain is required for toxin delivery.

Figure 6.

A) CdiA deletions and Cys substitutions. Deleted residues are indicated below domain identifiers. B - F) Cells expressing the indicated CdiA effectors were mixed with targets and incubated with maleimide-dye. CdiA proteins were analyzed by anti-TPS immunoblotting. G) FHA-2 localizes to the outer membrane after export. Cells expressing Cys1959 CdiA were mixed with tsx+ targets, then treated with dye and proteinase K. The sample from lane 4 was run on a sucrose gradient, and fractions analyzed by anti-TPS and anti-OmpF/C immunoblotting. H) Cells expressing Cys1959 ΔFHA2 were treated and analyzed as in panel G.