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. 2018 Dec 7;15(12):1499–1511. doi: 10.1080/15476286.2018.1553481

Figure 4.

Figure 4.

LINC00673 depletion induces a senescence-like phenotype. (A) SA-β-Gal accumulated in A549 cells 4 days after LINC00673 knockdown with siPOOLs (3 nM). As a positive control for staining, cells were treated with 200 nM doxorubicin. At least 200 cells were counted per condition (n = 4). The scale bar represents 100 µm. (B) SA-β-Gal staining of IMR-90 cells as in A (n = 3). (C) Cell cycle and E2F1-regulated genes were determined by RT-qPCR at 48 h after LINC00673 knockdown with siPOOLs (3 nM) in A549 cells. GAPDH was used as reference gene (n = 3). (D) SASP gene expression in A549 cells was determined by RT-qPCR as in C (n = 3). (E) Western Blot analysis 48 h after LINC00673 knockdown with siPOOLs (3 nM) in A549 cells. Specific antibodies were used for indicated proteins. One representative Western Blot is shown (n = 3). All data is shown as mean + SEM and the statistical significance was determined per two-sided unpaired Student t test, with *, P < 0.05; **, P < 0.01; ***, P < 0.001.