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The transcriptional levels of NDRG1 were inhibited by overexpressing NDRG1-OT1. MCF-7 cells were transfected with NDRG1-OT1 (2 μg) for 24 h, and NDRG1 (2 μg) for another 24 h. The expression levels of NDRG1-OT1 and NDRG1 were measured in MCF-7 cells by qRT-PCR. The relative expression level was normalized to empty control. Internal control: 18s. Data were repeated at least 3 times, and the results shown are the means ± SDs. *: P < 0.05 versus empty control.
