Figure 6.

Verification of NDRG1-OT1 interaction with HNRNPA1, HIF-1α, and KHSRP using RNA pull-down assays followed by western blotting. (a) Bioinformatics prediction of docking between NDRG1-OT1 and HNRNPA1, HIF-1α, and KHSRP, respectively. The 3D structure of NDRG1-OT1 was modeled by RNAComposer [52]. Color corresponds to each fragment. 2nd quarter: green; 3rd quarter: red; 4th quarter: cyan. The 3D structure of proteins (purple) was modeled by Phyre2 [35]. The docking prediction was performed by HDOCK [36]. (b) Western blots of HNRNPA1, HIF-1α, and KHSRP after RNA pull-down assays using full length (1–508 nt) NDRG1-OT1 as the probe. (c) Proposed model of NDRG1-OT1 interacting with various proteins to regulate the transcription of NDRG1 under hypoxia.