Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Antibody | Mouse monoclonal anti-TUBB3 (Tuj1) | Covance | MMS-435P; RRID:AB_2313773 | (1:1000) IF |
Antibody | Rabbit polyclonal anti-DNMT3A (H-295) | Santa Cruz Biotech | sc-20703; RRID:AB_2093990 | (1:1000) WB, (1:200) IF |
Antibody | Chicken polyclonal anti-GFP | Abcam | ab13970; RRID:AB_300798 | (1:1000) IF |
Antibody | rabbit polyclonal anti-HSP90 | Cell signaling | 4874; RRID:AB_2121214 | (1:1000) WB |
Antibody | rabbit polyclonal anti-H3 | Cell signaling | 9712 | (1:10000) WB |
Other | Dapi | ThermoFisher Scientific | D1306 | 1 ug/ml |
Genetic reagent (M. musculus, female) | C57BL/6J | The Jackson Laboratory | 000664; RRID:IMSR_JAX:000664 | |
Genetic reagent (M. musculus, male) | Mapt-tm1(EGFP)Klt | The Jackson Laboratory | 004779 | Homozygous TauEGFP |
Strain, strain background (M. musculus) | Dnmt3a fl/fl | PMID: 15215868,25130491,15757890 | ||
Strain, strain background (M. musculus) | Dnmt3b fl/fl | PMID: 15215868,25130491,15757890 | ||
Commercial assay or kit | DNeasy Blood and Tissue Kit | Qiagen | 69506 | |
Commercial assay or kit | MethylCode Bisulfite Conversion Kit | Invitrogen | MECOV50 | |
Software, algorithm | Methylpy | PMID: 26030523 | ||
Software, algorithm | Bowtie2 | PMID: 22388286 | RRID:SCR_016368 | |
Software, algorithm | STAR | PMID: 23104886 | RRID:SCR_015899 | |
Software, algorithm | edgeR | PMID: 19910308 | RRID:SCR_012802 | |
Software, algorithm | DSS | PMID: 24561809 | RRID:SCR_002754 |