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. 2019 Jan 15;8:e40197. doi: 10.7554/eLife.40197

Key resources table.

Reagent type (species)
or resource
Designation Source or reference Identifiers Additional
information
Antibody Mouse monoclonal anti-TUBB3 (Tuj1) Covance MMS-435P; RRID:AB_2313773 (1:1000) IF
Antibody Rabbit polyclonal anti-DNMT3A (H-295) Santa Cruz Biotech sc-20703; RRID:AB_2093990 (1:1000) WB, (1:200) IF
Antibody Chicken polyclonal anti-GFP Abcam ab13970; RRID:AB_300798 (1:1000) IF
Antibody rabbit polyclonal anti-HSP90 Cell signaling 4874; RRID:AB_2121214 (1:1000) WB
Antibody rabbit polyclonal anti-H3 Cell signaling 9712 (1:10000) WB
Other Dapi ThermoFisher Scientific D1306 1 ug/ml
Genetic reagent (M. musculus, female) C57BL/6J The Jackson Laboratory 000664; RRID:IMSR_JAX:000664
Genetic reagent (M. musculus, male) Mapt-tm1(EGFP)Klt The Jackson Laboratory 004779 Homozygous TauEGFP
Strain, strain background (M. musculus) Dnmt3a fl/fl PMID: 15215868,25130491,15757890
Strain, strain background (M. musculus) Dnmt3b fl/fl PMID: 15215868,25130491,15757890
Commercial assay or kit DNeasy Blood and Tissue Kit Qiagen 69506
Commercial assay or kit MethylCode Bisulfite Conversion Kit Invitrogen MECOV50
Software, algorithm Methylpy PMID: 26030523
Software, algorithm Bowtie2 PMID: 22388286 RRID:SCR_016368
Software, algorithm STAR PMID: 23104886 RRID:SCR_015899
Software, algorithm edgeR PMID: 19910308 RRID:SCR_012802
Software, algorithm DSS PMID: 24561809 RRID:SCR_002754