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. 2019 Jan 3;8:e40063. doi: 10.7554/eLife.40063

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© 2019, Miller et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 3. — (A) Equalized cell wall fractions of M. tuberculosis H37Rv, ΔsecA2, ΔsatS and complemented (ΔsatS +psatS_Mtb) strains were analyzed by immunoblot using Mce1A, Mce1E, and 19 kDa antibodies to monitor differences in protein levels. (B) Equalized M. smegmatis mc²155, ΔsecA2, ΔsatS, and ΔsatS +psatS_Msm cell wall fractions were analyzed by immunoblot using Mce1D, HA (Mce4A-HA), and MspA antibodies. (C) Equalized protein from whole cell lysates of M. tuberculosis H37Rv, ∆secA2, ∆satS and the complemented strain (∆satS +psatS_Mtb) were immunoblotted for Mce1A, Mce1E, and 19 kDa. (D) Equalized cell wall fractions of M. tuberculosis H37Rv, ΔsecA2, and ΔsatS strains were analyzed by immunoblot using PknG, PhoS1, and 19 kDa antibodies. (E) Equalized M. smegmatis mc²155, ΔsecA2, and ΔsatS, cell wall fractions were analyzed by immunoblot using HA (Msmeg1704-HA) and MspA antibodies. Densitometry of blots from three experiments was performed (ImageJ). Percent difference of the mean intensity relative to wild-type is reported below each immunoblot. *, p<0.05 by ANOVA and Tukey’s post hoc test.