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. 2018 Sep 10;15(2):242–258. doi: 10.1080/15548627.2018.1515530

Figure 2.

Figure 2.

Increased LAMP1 expression mediates the formation of CagA-degrading autolysosomes. (a) AGS cells were transfected with pTet-On and TRE2hyg-mCherry-EGFP-LC3B plasmids, infected with H. pylori for 5 h (MOI 50), incubated in a medium containing antibiotic for 24 h, and then, immunostained for LAMP1. Scale bar: 20 μm. (b) AGS cells, which had been transfected with pTet-On and TRE2hyg-mCherry-EGFP-LC3B plasmids, were transfected with control, LAMP1 siRNA-1, or LAMP1 siRNA-2, infected with H. pylori for 5 h (MOI 50), and incubated in a medium containing antibiotic for 24 h. Thereafter, autophagosomes (yellow puncta) and autolysosomes (red puncta) were counted. Scale bar: 20 μm. Data are presented as the mean ± SD of 3 independent assays. **P < 0.01; NS, not significant. (c) AGS cells transfected with control, LAMP1 siRNA-1, or LAMP1 siRNA-2, had been infected with H. pylori for 5 h (MOI 50), and incubated in a medium containing antibiotic for the indicated times. Data are presented as the mean ± SD of 3 independent assays. *P < 0.05.