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. 2018 Sep 10;15(2):242–258. doi: 10.1080/15548627.2018.1515530

Figure 4.

Figure 4.

CAPZA1 binds to LRP1-ICD during autophagy induction. (a) AGS cells were infected with H. pylori for 5 h (MOI 50) and incubated in a medium containing antibiotic for 24 h. Then, an immunoprecipitation assay was performed on these cells with an anti-LRP1-carboxyterminal end antibody. The immunoprecipitate was subjected to SDS-PAGE, the gel was stained with a silver stain, and then 2 proteins (protein band 1 and 2) were identified by HPLC-Chip-MS/MS experiments using an Agilent 1100 LC/MSDTrap-XCT series system. (b) AGS cells were infected with H. pylori for 5 h (MOI 50) and incubated in a medium containing antibiotic for 0 and 24 h. Then, subcellular fractionation of these cells indicated the localization of GTF2I and CAPZA1 in cell membrane (Mem), cytoplasmic (Cyt) and nuclear (Nuc) extracts. (c) AGS cells were infected with H. pylori for 5 h (MOI 50) and incubated in a medium containing antibiotic for 0 and 24 h. After subcellular fractionation of these cells, an immunoprecipitation assay was performed with an anti-LRP1-carboxyterminal end antibody or an IgG from rabbit serum.