CAPZA1 and GTF2I function as negative regulators of LAMP1 expression. (a) AGS cells were transfected with control siRNA, CAPZA1 siRNA-1, or CAPZA1 siRNA-2 and incubated for the indicated durations. (b) AGS cells were transfected with control siRNA, CAPZA1 siRNA-1, or CAPZA1 siRNA-2, infected with H. pylori for 5 h (at MOI 50), and incubated in a medium containing antibiotic for the indicated time periods. Data are presented as the mean ± SD of 3 independent assays. *P < 0.05, NS, not significant. (c) AGS cells were transfected with control siRNA, GTF2I siRNA-1, or GTF2I siRNA-2 and incubated for the indicated durations. (d) AGS cells were transfected with control siRNA, CAPZA1 siRNA-1, CAPZA1 siRNA-2, GTF2I siRNA-1, or GTF2I siRNA-2, infected with H. pylori for 5 h (at MOI 50), and incubated in a medium containing antibiotic for the indicated time periods. The mRNA expression of LAMP1 was quantified by real-time qRT-PCR. Data are presented as the mean ± SD of 3 independent assays. *P < 0.05, **P < 0.01. (e) AGS cells were transfected with PCMV-control or pCMV-CAPZA1 plasmid, infected with H. pylori for 5 h (at MOI 50), and incubated in a medium containing antibiotic for 24 h. Then, the mRNA expression of LAMP1 was quantified by real-time qRT-PCR. Data are presented as the mean ± SD of 3 independent assays. *P < 0.05.