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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Cytotherapy. 2018 Nov 27;21(1):76–82. doi: 10.1016/j.jcyt.2018.10.009

Figure 1. Comparison between continuous and intermittent fluidic cell culture conditions.

Figure 1.

The proliferative capacity of primary T-cells was compared in a continuous perfusion setup and intermittent perfusion setup. Continuous perfusion was performed at 100mL/min using a magnetically actuated pump while intermittent perfusion was performed every other day (starting at Day 3) at 100mL/min for 5 minutes. A) We observe a 0.25× change in the continuous group, 1.3× in intermittent, and 13× in flask culture. It is clear that continuous perfusion is detrimental to T-cell proliferation. Interestingly, intermittent perfusion is only modestly better and significantly worse than standard T-flask culture – this difference may arise due to the material properties of the different culture vessels. ** = P<0.001 B) We observe the ability of shearing action to dissociate T-cell blast clusters. We were able to visually and microscopically identify clusters, whereby after 5 minutes of continuous flow, we find a near homogenous single cell suspension.