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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Cytotherapy. 2018 Nov 27;21(1):76–82. doi: 10.1016/j.jcyt.2018.10.009

Figure 2. Comparison of cell culture vessels using a Jurkat T cell line.

Figure 2.

We herein assessed the growth characteristics and media properties in a standard tissue culture flask and silicone, polyolefin/EVA, and FEP cell culture bags. A) Comparability between flask/silicone and polyolefin/FEP is observed wherein the flask/silicone has significantly improved cell proliferative capacity. Both flasks and silicone bags were significantly different compared to polyolefin/EVA and FEP (*** = P<0.001). B) Comparison of several media characteristics including: dissolved O2, CO2, pH, and HCO3. Comparability is seen in O2, with the flask being overall lower across all days. CO2 demonstrates an advantage to silicone with overall lower values across the experiment. pH values show comparability with an overall downward trend likely due to increased cell burden. HCO3 demonstrates drastic difference at early time points but normalizes by the end of the culture. * = P<0.05, ** = P<0.01, *** = P<0.005.