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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Cytotherapy. 2018 Nov 27;21(1):76–82. doi: 10.1016/j.jcyt.2018.10.009

Figure 3. Growth comparison of primary human T-cells.

Figure 3.

Proliferative comparison of flask versus silicone bag for the growth of healthy, primary T-cells. A) Picture depicting the custom designed and manufactured silicone bag. Tan loop represents the portion interfacing with the peristaltic pump for flowing action. Medium introduction and sampling were accomplished through a needleless syringe port. B) Growth comparison over a 9-day culture. Medium and fresh IL-2 was added on the days 3, 5, 7, and 9. Flask growth holds an advantage at early time points, however, silicone systems show a drastic increase in cell yield by the latest time point. * = P<0.05. C) Growth comparison over a 9day culture as measured by cell concentration. We observe that the bag has an increasing cell concentration at each time point whereas flask conditions spike and decrease. Note that cell concentration was normalized to 1M/mL on days 3, 5, 7, and 9.