Figure 4. Shrinkage of splenic white pulps with intact red pulps in Rag-2^−/− mice. H&E stained sections of spleens were examined for microscopic changes. (A–C) Cross sections of wild-type (WT) spleen (A) shows the white pulp (WP) and red pulps (RP), while WPs in the spleen of Rag-2^−/− became less abundant and smaller (B and C). Scale bars; 500 µm. (D–F) Compared to a dense population of lymphocytes in WT WP (D), Rag-2^−/− mice (E and F) show an evident decrease with an increased number of megakaryocytes (arrowheads). Scale bars; 5 µm. (G) Quantification of area confirmed the reduction of WPs in Rag-2^−/− mice (dark gray bars) in comparison with WT (black bars), while RPs remained unchanged. ^***P<0.001.