Figure 3. Immunohistochemistry for doublecortin (DCX) in the dentate gyrus of control, vehicle-treated, and Bacopa monnieri extract (BME)-treated mice. In the BME-treated group, DCX immunoreactive neuroblasts and their dendrites are most abundantly observed in the dentate gyrus. GCL, granule cell layer; ML, molecular layer; PL, polymorphic layer. Scale bar= 50 µm. The relative optical densities (RODs) expressed as a percentage of the value representing the DCX immunoreactivity in the dentate gyrus of the control group are shown. The number of DCX-immunoreactive neuroblasts in the dentate gyrus per section for each group are also shown (n=5 per group; ^aP<0.05, vs. control group; ^bP<0.05, vs. vehicle-treated group). Data are presented as mean±SEM.