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. 2019 Jan 9;9:3276. doi: 10.3389/fmicb.2018.03276

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Copyright © 2019 Wei, Wang, Alper, Xu, Knoshaug, Van Wychen, Lin, Luo, Decker, Himmel and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Plasmid construction for cellulase expression in Yarrowia lipolytica. (A) Plasmid construct 162 with CBH I-CBH II-EG II cloned in vector pUC57. (B) Plasmid construct 165 with CBH I-CBH II-EG II cloned in the Y. lipolytica expression vector pYLEX1, which carries Leu2 as a selection marker. More details are described in the Materials and Methods. Symbols and abbreviations: rep (pMB1), origin of replication derived from plasmid pMB1; t, terminator; ^{∗, ∗∗}, and ^∗∗∗ indicate the relatively low, middle and high expression levels of promoters, respectively.