Table 1.
Yarrowia lipolytica strains and plasmids.
| Strain, plasmids, or constructs | Description: (strain genotype and phenotype; cloned genes and MW of encoded proteins) | Source |
|---|---|---|
| Parent and derivative strains | ||
| Po1f | Genotype: MatA, leu2-270, ura3-302, xpr2-322, axp-2; Phenotype: Leu-, Ura-, ΔAEP, ΔAXP, Suc+ | Madzak et al., 2000 |
| Po1g | Genotype: MatA, leu2-270, ura3-302::URA3, xpr2-332, axp-2; Phenotype: Leu-, ΔAEP, ΔAXP, Suc+, pBR platform | Madzak et al., 2000 |
| YL101 | Previously named as Yl (EG II); expressing Tr eg2 (P07982; 42 kDa) with a hybrid promoter (hp4d) in parent strain Po1g. | Wei et al., 2014 |
| YL102 | Previously named as Yl (CBH II); expressing Tr cbh2 (P07987; 47 kDa) with a hybrid promoter (hp4d) in parent strain Po1g. | Wei et al., 2014 |
| YL151 | Previously named as Yl (chimeric CBH I); expressing chimeric cbh1 (Te CBH1 catalytic domain-Tr Linker-Tr CBM1; AAL89553 and P62694; 53 kDa) with a hybrid promoter (hp4d) in parent strain Po1g. | Wei et al., 2014 |
| HA1 | Genotype: Po1f Δpex10 Δmfe1 leu- ura+ DGA1; Phenotype: prevent peroxisome biogenesis and β-oxidation by Δpex10 and Δmfe1, respectively; enhance lipid synthesis by DGA1 overexpression. | Blazeck et al., 2014 |
| YL165-1 | Genotype: Po1f Δpex10 Δmfe1 leu- ura+ DGA1 chimeric cbh1 cbh2 eg2; Phenotype: prevent peroxisome biogenesis and β-oxidation by Δpex10 and Δmfe1, respectively; enhance lipid synthesis by DGA1 overexpression; co-express CBH I (with TEFin promoter) – CBH II (with GPD promoter) – EG II (with EXP1 promoter). | This study |
| Plasmids and the cloned genes | ||
| pYLEX1 (i.e., pINA1269) | hybrid promoter (hp4d); selection marker gene (LEU2). | Madzak et al., 2000 |
| pYLSC1 (i.e., pINA1296) | hybrid promoter (hp4d); secretion signal (XPR2 pre-region); selection marker gene (LEU2). | Madzak et al., 2000 |
| pNREL151 | Chimeric cbh1 (Te CBH1 catalytic domain-Tr Linker-Tr CBM1; AAL89553 and P62694; 53 kDa) in SfiI/Xbal cut pYLSC1. | Wei et al., 2014 |
| pNREL162 | Chimeric cbh1-cbh2-eg2 cassette; cloned in vector pUC57. | This work |
| pNREL165 | Chimeric cbh1-cbh2-eg2 cassette; cloned in vector pYLEX1. | This work |
The theoretical molecular weight (MW) was calculated based on amino acid sequence (without a signal peptide). CBH, cellobiohydrolase; EG, endoglucanase; Te, Talaromyces emersonii; Tr, T. reesei.