Table 2.
Enzyme activity of supernatant or concentrated crude enzymes of transformants co-expressing CBH I-CBH II-EG II.
| Enzyme-Avicel incubation time (50°C) | Y. lipolytica strain | 1× supernatant enzyme activity |
35× concentrated crude enzyme activity |
||
|---|---|---|---|---|---|
| Total Glu equiv. released g L-1 | % Avicel to Glu equiv. | Total Glu equiv. released g L-1 | % Avicel to Glu equiv. | ||
| 0 h | HA1 (EV) | 0 | 0 | 0 | 0 |
| YL165-1 | 0 | 0 | 0 | 0 | |
| YL165-5 | 0 | 0 | 0 | 0 | |
| YL165-7 | 0 | 0 | 0 | 0 | |
| 1 h | HA1 (EV) | 0 | 0 | 0 | 1% |
| YL165-1 | 0 | 0 | 1.56 | 31% | |
| YL165-5 | 0 | 0 | 1.19 | 24% | |
| YL165-7 | 0 | 0 | 0.63 | 13% | |
| 24 h | HA1 (EV) | 0 | 0 | 0.04 | 1% |
| YL165-1 | 0.30 | 6% | 2.78 | 56% | |
| YL165-5 | 0.24 | 5% | 2.48 | 50% | |
| YL165-7 | 0.14 | 3% | 1.34 | 27% | |
| 5 days | HA1 (EV) | 0.00 | 0% | 0.11 | 2% |
| YL165-1 | 0.53 | 11% | 3.45 | 69% | |
| YL165-5 | 0.50 | 10% | 3.28 | 66% | |
| YL165-7 | 0.26 | 5% | 1.55 | 31% | |
Data presented were the average of three biological replicates and the SEM (standard error of the mean) was less than 10%. Glu equiv., glucose equivalent. HA1 (EV), parent control strain HA1 transformed with empty vector.