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. 2019 Jan 9;5:131. doi: 10.3389/fnut.2018.00131

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Copyright © 2019 de Aquino, Leitão, Oliveira Alves, Coelho-Santos, Guerrant, Ribeiro, Malva, Silva and Oriá.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of regional basic (RBD) and high-fat (HFD) diets on blood-brain barrier properties. An increase of albumin (A) and a decrease of claudin-5 (B) protein levels were observed with both RBD and HFD diets. (C) Regarding caveolin-1 (Cav1) protein levels, there was also a significant increase of its total levels in HFD group. (D) Representative immunohistochemistry images for Collagen IV (green, brain vessels), and Hoechst 33342 (blue, nuclei). Scale bar = 40 μm. (E) DRB and HFD induced a significant decrease in collagen IV immunoreactivity. Moreover, both diets induced a significant upregulation in (F) VCAM-1 (vascular cell adhesion molecule-1). Above the bars, representative western blot images of Albumin (66 kDa), claudin-5 (25 kDa), caveolin-1 (20 kDa), VCAM-1 (110 kDa), and GAPDH (37 kDa) are shown. Data were analyzed by using Kruskal-Wallis test, followed by Dunn's post-test for multiple comparisons, n = 5–7. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. nourished control group (CTR). The results are shown as mean ± SEM.