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. 2019 Jan 9;9:1906. doi: 10.3389/fphys.2018.01906

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Copyright © 2019 Ye, An, Jiang, Ding, Shang, Christiaens, Taning, Smagghe, Niu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative expression of hunchback upon different dshunchback doses and time-points after injection in Acyrthosiphon pisum. (A) Aphids were injected with 6, 60, 600, and 1200 ng, respectively. RNA was extracted after 36 h. (B) Silencing efficiency was detected at different sampling time-points (0, 12, 24, 48, 72, and 96 h) after the injection of 600 ng of dsRNA. Water and dsGFP was injected in the blank and negative control, respectively. RNA was extracted at each time-point. Each treatment contained four replicates analyzed by qPCR and four individuals were pooled in each replicate. Lower-case letters above each bar indicate significant differences among different treatments using analyses of one-way analysis of variance followed by Tukey’s test (mean ± SE; P < 0.05).