Skip to main content
. 2019 Jan 9;9:3308. doi: 10.3389/fmicb.2018.03308

FIGURE 2.

FIGURE 2

RHDV-NSP6 influenced cell growth and apoptosis of RK13 cells. (A,B) Cell viability analysis of RK13 cells. RK13 cells transfected with pcDNA3.1-NSP6 (5000 ng) was detected by MTT assay (A) and trypan blue dye exclusion assay (B) at the indicated time points post transfection. Empty vector transfected RK13 cells were used as control. (C) Cell apoptosis was measured by flow cytometry analysis. Results are expressed as scatter diagram (left) and calculated percentage of annexin-V-positive cell population (right). (D) TUNEL labeling of NSP6-transfected RK13 cells. Empty vector and NSP6-transfected RK13 cells fixed at 24 hpt were labeled with TUNEL (green) and then counterstained with DAPI (blue). NSP6 expression was also determined by immunofluorescence using FITC-conjugated murine anti-His monoclonal antibodies (green). Scale, 50 μm. Results are from one representative of three independent experiments. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.