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. 2019 Jan 9;9:3308. doi: 10.3389/fmicb.2018.03308

Table 1.

Sequences of primers used for amplification of NSP6 and the generation of NSP6 point mutants.

Name Sequence
NSP6-Fwd 5′-GCGGAATTCCACCATGGGCCTACCTGGTTTTATGAG-3′
NSP6-Rev 5′-GCGAAGCTTCATTACACCATCACAAAGGG-3′
H27N -Fwd 5′-CTCCAACACCCATACTGCCAGGTCAAGTTGCTCAGAGATT-3′
H27N -Rev 5′-CTGGCAGTATTGGTGTTGGAGATGTACAGTCCGTTCCCTA-3′
D44G -Fwd 5′-CCCACCACTGGCCTGTGCCTTGTCAAGGGCGAGACAATCC-3′
D44G -Rev 5′-AAGGCACAGGCCAGTGGTGGGTGAGCATGTGACAATCTCT-3′
C104G -Fwd 5′-CCACGGGGACGGTGGACTACCGTTGTATGACTCTAGTGGG-3′
C104G -Rev 5′-GGTAGTCCACCGTCCCCGTGGGTGGTCTGCGTGCAACCAT-3′