Table 7.
Effect of experimental diets on immune response (TAS, μmol g-1 organ; SOD, U mg-1 protein; PO, O.D.490 nm; MDA, nmol mg-1 protein; carbonyl protein, nmol mg-1 protein) of L. vannamei both during the 8 weeks rearing period and after the WSSV injection challenge test.
| Diets | Trial1: During the 60-day rearing period |
Trial 2: After the challenge test |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| D1 | D2 | D3 | D4 | D5 | D1 | D2 | D3 | D4 | D5 | |
| Hepatopancreas antioxidant parameters | ||||||||||
| TAS | 2.34 ± 0.04 aB | 4.20 ± 0.03 bB | 4.22 ± 0.05 bB | 4.21 ± 0.02 bB | 4.20 ± 0.05 bB | 1.97 ± 0.04 aA | 3.18 ± 0.02 cA | 3.19 ± 0.01 cA | 2.55 ± 0.05 bA | 3.21 ± 0.03 cA |
| SOD | 2.27 ± 0.04 A | 2.29 ± 0.09 A | 2.32 ± 0.04 A | 2.29 ± 0.07 A | 2.30 ± 0.02 A | 4.22 ± 0.03 cB | 3.14 ± 0.02 aB | 3.15 ± 0.04 aB | 3.34 ± 0.06 bB | 3.14 ± 0.02 aB |
| PO | 5.16 ± 0.04 aA | 6.78 ± 0.06 bA | 6.82 ± 0.07 bA | 6.79 ± 0.05 bA | 6.80 ± 0.05 bA | 7.38 ± 0.07 aB | 10.26 ± 0.04 cB | 10.24 ± 0.04 cB | 9.61 ± 0.08 bB | 10.21 ± 0.18 cB |
|
Hepatopancreas oxidative injury parameters | ||||||||||
| MDA | 12.33 ± 0.09 A | 12.27 ± 0.10 A | 12.26 ± 0.07 A | 12.26 ± 0.05 A | 12.26 ± 0.04 A | 32.26 ± 1.08 bB | 21.54 ± 0.47 aB | 21.89 ± 0.23 aB | 21.97 ± 0.44 aB | 22.55 ± 0.23 aB |
| Carbonyl | 1.22 ± 0.02 A | 1.19 ± 0.05 A | 1.21 ± 0.04 A | 1.22 ± 0.03 A | 1.19 ± 0.04 A | 2.90 ± 0.04 cB | 1.70 ± 0.03 aB | 1.71 ± 0.04 aB | 2.19 ± 0.02 bB | 1.69 ± 0.03 aB |
| protein | ||||||||||
Values are means ± SEM of three replicates. The superscript small letters (a,b,c) in the same row mean the significant difference at P < 0.05 within the same trial. The superscript capital letters (A,B) in the same row mean the significant difference at P < 0.05 of the same dietary treatment between trial 1 and trial 2 (such as comparison of P0 between trial 1 and trial 2).