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. 2019 Jan 9;11:484. doi: 10.3389/fnmol.2018.00484

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Copyright © 2019 Kuramochi and Doi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Localization patterns of the AMPA-type glutamate receptor and the glutamate-gated chloride ion channel on AIB neurites. (A) Left: schematic cartoon showing the morphology of AIBL neurons, modified from worm atlas (http://www.wormatlas.org). Each arrow indicates the direction for frontal-view observation or side-view observation, respectively. Right: blue and yellow lines indicate the AIBL or AIBR neurite respectively. From left-side view, both the proximal AIBL neurite and the distal AIBR neurite can be clearly observed. (B) The localization patterns of the AMPA-type glutamate receptor on the AIB neurites. The GFP-fused GLR-1 is expressed specifically in the AIB neurons indicated by the mCherry marker. (C) The localization patterns of the glutamate-gated chloride ion channel on the AIB neurites. The Venus-fused GLC-3 is expressed in the AIB neurons indicated by the mCherry marker. (D) One-side images for the localization of the GLR-1::GFP fusion protein in AIB neurons. Left images show the localization patterns of the fluorescent proteins in both the AIBL proximal neurite and AIBR distal neurite from each neuronal cell body. Right images show the localization patterns of the fusion proteins in both the AIBR proximal neurite and the AIBL distal neurite from each neuronal cell body. Open and closed arrowheads mark each distal or proximal neurite, respectively. (E) The averaged fluorescence intensity of the GLR-1::GFP fusion protein in the proximal or distal region of AIB neurons, respectively. ***p < 0.001, Wilcoxon rank sum test (n = 11). (F) One-side images for the localization patterns of the GLC-3::GFP fusion protein in AIB neurons. Right images show the localization patterns of the fluorescent proteins in both the AIBR proximal neurite and AIBL distal neurite from each neuronal cell body. Left images show the localization patterns of the fusion proteins in both the AIBL proximal neurite and the AIBR distal neurite from each neuronal cell body. Open and closed arrowheads mark each distal or proximal neurite, respectively. (G) The averaged fluorescence intensity of GLC-3::Venus fusion protein in the proximal or distal region of AIB neurons. ***p < 0.001, Wilcoxon rank sum test (n = 13).