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. 2019 Jan 9;9:710. doi: 10.3389/fgene.2018.00710

FIGURE 4.

FIGURE 4

Multiple alignment of norA sequences of the three norA alleles, from selected Portuguese MRSA clinical isolates (SM) and from strains of representative clonal lineages. The MRSA strains displayed were selected to represent the main clones found, namely SM10 – CC5; SM3 – CC22; MW2 – CC1; Newman – CC8; MRSA252 – CC30. The Shine-Dalgarno (SD) sequence is underlined in black. The shaded regions correspond to the putative TMS of NorA as suggested by Paulsen et al. (1996). The aminoacid regions inside the boxes are relative to the conserved motifs described for MFS transporters with 12 TMS. The black residues shown above the main polypeptide sequence represent the variations present in the various NorA polypeptide sequences relatively to the NorAI variant. The red residues represent alterations found among the SM collection of Portuguese MRSA clinical isolates. The red boxes indicate the primers described in Table 2 for distinction of norA alleles.