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. 2019 Jan 9;9:710. doi: 10.3389/fgene.2018.00710

FIGURE 5.

FIGURE 5

Multiple alignment of NorA polypeptide sequences derived from alleles norAI (Yoshida et al., 1990), norAII (Noguchi et al., 2004), and norAIII (Kaatz et al., 1993) and from the norA alleles from selected S. aureus strains. The MRSA strains displayed were selected to represent the main S. aureus clonal lineages found for the strains with genomes publicly available and for the Portuguese strains (SM10 – CC5; SM39 – CC88). Sequences are grouped according to the respective norA allele (orange – norAI; green – norAII; blue - norAIII; yellow – norA-CC59/121). The lines correspond to the transmembrane segments (TMS) of NorA, as predicted by Paulsen et al. (1996) (green), by the HMMTOP method as presented in Bhaskhar et al. (2016) (red) and as predicted by the Phyre2-based NorA model (purple). The aminoacid regions inside the boxes are relative to the conserved motifs described for MFS transporters with 12 TMS (Paulsen et al., 1996). The red box corresponds to the amino acid substitution likely to affect NorA function.