Table 1.
Primers used to sequence the norA promoter and coding region of Staphylococcus aureus clinical strains.
| Primer | Sequence(5′ – 3′) | Amplicon size | Locationa | Reference |
|---|---|---|---|---|
| norA_fw(a) | TGTTAAGTCTT GGTCATCTGCA | 761 | 706005–706026 | Couto et al., 2008 |
| norA_rv(a) | CCATAAATCCA CCAATCCC | 706765–706747 | This studyb | |
| norA_fw(b) | TTCACCAAGCC ATCAAAAAG | 620 | 706671–706690 | Sierra et al., 2000 |
| norA_rv(b) | CTTGCCTTTCT CCAGCAATA | 707290–707271 | Couto et al., 2008 | |
| norA_fw(c) | GGTCATTATTA TATTCAGTTGTTG | 419 | 707135–707158 | Schmitz et al., 1998 |
| norA_rv(c) | GTAAGAAAAAC GATGCTAAT | 707553–707534 | ||
aPosition of the primers in the genome of S. aureus DSM20231 (accession no. CP011526.1). bThe primer was designed with the aid of the free software Primer3 (http://primer3.sourceforge.net/) and tested in silico (http://insilico.ehu.es/PCR/).