Table 2.
List of primers proposed to amplify and differentiate the norA alleles by conventional PCR.
| Primer | Sequence(5′ – 3′) | Amplified norA allele | Amplicon size (nt) | Reference |
|---|---|---|---|---|
| norA_fw(b) | TTCACCAAGC CATCAAAAAG | all | 704 | Sierra et al., 2000 |
| NorA2 | GCACATCAAA TAACGCACCT | |||
| YonorA | ATATTCAGTTGT TGTCTTAATAT | norAI | 230 | Sierra et al., 2000 |
| NorA2 | GCACATCAAA TAACGCACCT | |||
| NorAII | CTGTATTCTTT ATATACATCG | norAII | 391 | This studya |
| NorA2 | GCACATCAAAT AACGCACCT | Sierra et al., 2000 | ||
| NorAIII | GACCCTAAAA AAGTTTCGAC | norAIII | 526 | This studya |
| NorA2 | GCACATCAAATA ACGCACCT | norA-CC59/CC121 | Sierra et al., 2000 | |
| Plus HindIII restriction: | ||||
| norAIII: 166 nt + 360 nt; | ||||
| norA-CC59/121: no cut (526 nt) | ||||
aThe primers were designed with the aid of the free software Primer3 (http://primer3.sourceforge.net/). The primers and the HindIII restriction were tested in silico (http://insilico.ehu.eus/) against several S. aureus genome sequences of representative clonal lineages.