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. 2019 Jan 9;9:1928. doi: 10.3389/fpls.2018.01928

Figure 4.

Figure 4

DNA cleavage during aerenchyma formation in H. annuus stems. Genomic DNA was isolated from control (lane 1) and waterlogged seedlings after 1D (Lane 2), 2D (Lane 3), 3D (Lane 4), and 4D (Lane 5), stained with ethidium bromide, and electrophoretically separated. Lanes 1 and 2. DNA ladder was not detected. Lanes 3 and 4: Clear DNA smearing present on the second and third day after WA treatment, it shows a number of cells undergo PCD in thses stages; Lane 5: Light DNA smearing present on the fifth day after WA treatment. Consistent results were obtained in three independent extracted DNA samples. Marker: 2000 bp.