Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jan 15;10:217. doi: 10.1038/s41467-018-08140-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Endogenous Chi3l3 expression in the adult SVZ during EAE. a Chi3l3 gene expression in the subventricular zone (SVZ) of healthy control mice (HC; n = 8), preclinical EAE (6 days post immunization (dpi; n = 6), EAE onset (13dpi; n = 6), acute EAE (14 dpi; n = 6), initial recovery (18 dpi; n = 8) and chronic EAE (36 dpi, n = 6) measured by qRT-PCR (multiple t -tests with Dunn–Bonferroni’s multiple comparison test). b–e Confocal images of the SVZ of representative healthy controls (HC), acute EAE mice (12–14 dpi). Sections were immunostained for the nuclear marker TO-PRO-3 (blue), Chi3l3 (red) and the microglia/macrophage marker CD11b (green, b–e) and the lymphocyte common antigen CD45 (blue, d). Chi3l3 expression in the SVZ was only detected during acute EAE and was localized to CD11b-positive cells (yellow arrowheads) within the corpus callosum (CC) c and lesions d, where they co-expressed CD45 at high (yellow arrowhead) and low levels (white arrowhead). Dashed lines mark the wall of the lateral ventricle (LV). Scale bar, 200 μm. e Ortho-view images of representative Chi3l3⁺/CD11b⁺ double-positive cells exhibiting round (upper panel) and ramified (lower panel) morphology. (mean ± s.e.m. *p < 0.05; **p < 0.01; ***p < 0.005)