Fig. 7.

Human CLPs directly promote oligodendrogenesis. Human neural stem cells (NSCs) were cultured in the presence of PBS (control), human CHI3L1 and human CHIT1 at 250 ng/ml for 14 days. a–c Cells were immunostained for MBP (a, oligodendrocytes, green), DCX (a, neurons, red), NG2 (b, OPC, green), GFAP (c, astrocyte, red), SOX2 (d, NSC, red) and nuclear stain DAPI (blue). Scale bar, 50 μm. e Quantification of MBP⁺, NG2⁺, GFAP⁺, DCX⁺, and SOX2⁺ NSCs after exposure to human Chi3L1or human Chit1. Exposure of differentiating NSCs to CHI3L1 and CHIT1 led to significant increase in oligodendrogenesis. Values are expressed as percent change to PBS-treated NSCs (data are representative of three independent experiments. one-way ANOVA with Dunnett’s multiple comparison test; mean ± s.e.m *p < 0.05; **p < 0.01; ***p < 0.005; N.S. = not significant