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. 2019 Jan 15;5:3. doi: 10.1038/s41522-018-0075-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — M.tb PpiB protein enhances biofilm and pellicle formation in recombinant M.smegmatis: M. tb PpiA and PpiB genes were cloned in M.smegmatis, as described in methods, to generate Ms_PpiA and Ms_PpiB strains, respectively. M.smegmatis vector control (Ms_VC), Ms_PpiA and Ms_PpiB were grown in static culture and induced with anhydrotetracycline for expressing M.tb PPIases. a Pellicle formed in Ms_VC, Ms_PpiA and Ms_PpiB cultures from a representative experiment. The pellicle formed at the liquid air interface was quantified as described in methods. Values shown in b are mean [±s.e.m] from three independent experiments and depict the amount of biofilm formed in Ms_VC, Ms_PpiA and Ms_PpiB cultures. ***p < 0.005 vs. Ms_VC (Student’s t-test)