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. 2019 Jan 15;9:132. doi: 10.1038/s41598-018-36725-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cartoons of PFV IN and integration reaction products. (a) FL PFV IN is 392 amino acids. The truncation of NED (purple) and NTD (red) domains has the first 103 amino acids removed to generate ΔNTD. Truncation of the CTD (blue) deleted 73 amino acids. These truncation mutants preserved the PFV IN(K120E) and PFV IN(D273K) residues that direct monomers to the inner or outer positions of the intasome. (b) PFV intasomes catalyze the covalent joining of viral donor DNA oligomers to a supercoiled plasmid target DNA. Half site integration (HSI) is the joining of a single viral donor to the plasmid, generating a tagged circle. This integration reaction product has a nick at the point of joining that releases the supercoils. HSI products with fluorescently labeled viral donors include a single fluorophore. Concerted integration (CI) is more physiologically relevant and is the joining of two viral donors to the plasmid. This integration product relaxes to a linear product with the viral donor DNAs at the ends. There are two fluorophores present in CI products.